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Experimental design for the microdissection of equine ovarian follicles and end points evaluated. One ovary was harvested from mixed-breed mares ( n = 11) and sectioned into three ovarian portions (two lateral and one intermediary portion). The intermediary portion was further separated into three equally sized dorsal, medial, and ventral regions. The outermost cortical areas of these regions were sliced at 0.25 and 0.50 mm thicknesses using <t>a</t> <t>Stadie-Riggs</t> tissue slicer. All slices that contained visible preantral and antral follicles were subjected to microdissection to collect these follicles. The experiment was replicated 11 times, with each animal/ovary considered as one replicate. Microdissected follicles were evaluated for spatial distribution, viability, mitochondrial membrane potential, ROS, and histone trimethylation.
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Experimental design for the microdissection of equine ovarian follicles and end points evaluated. One ovary was harvested from mixed-breed mares ( n = 11) and sectioned into three ovarian portions (two lateral and one intermediary portion). The intermediary portion was further separated into three equally sized dorsal, medial, and ventral regions. The outermost cortical areas of these regions were sliced at 0.25 and 0.50 mm thicknesses using a Stadie-Riggs tissue slicer. All slices that contained visible preantral and antral follicles were subjected to microdissection to collect these follicles. The experiment was replicated 11 times, with each animal/ovary considered as one replicate. Microdissected follicles were evaluated for spatial distribution, viability, mitochondrial membrane potential, ROS, and histone trimethylation.

Journal: Reproduction & Fertility

Article Title: Characterization of isolated late preantral and early antral equine ovarian follicles

doi: 10.1530/RAF-25-0109

Figure Lengend Snippet: Experimental design for the microdissection of equine ovarian follicles and end points evaluated. One ovary was harvested from mixed-breed mares ( n = 11) and sectioned into three ovarian portions (two lateral and one intermediary portion). The intermediary portion was further separated into three equally sized dorsal, medial, and ventral regions. The outermost cortical areas of these regions were sliced at 0.25 and 0.50 mm thicknesses using a Stadie-Riggs tissue slicer. All slices that contained visible preantral and antral follicles were subjected to microdissection to collect these follicles. The experiment was replicated 11 times, with each animal/ovary considered as one replicate. Microdissected follicles were evaluated for spatial distribution, viability, mitochondrial membrane potential, ROS, and histone trimethylation.

Article Snippet: Each region generated four slices total: two 0.25 mm-thick slices were generated using an innovative, modified Stadie-Riggs tissue slicer (Federal University of Rio Grande; patent pending) and two 0.50 mm-thick slices were generated using a Stadie-Riggs tissue slicer (Thomas Scientific, USA).

Techniques: Laser Capture Microdissection, Membrane